Detection of enterotoxigenic Bacteroides fragilis by PCR
نویسندگان
چکیده
منابع مشابه
Detection of enterotoxigenic Bacteroides fragilis by PCR.
Strains of enterotoxigenic Bacteroides fragilis (ETBF) are associated with diarrhea in young farm animals and, at least in particular settings, in children. Enterotoxin production by ETBF is currently detected by a tissue culture assay with HT-29 cells. We have developed a PCR assay based on the detection of the enterotoxin gene to identify ETBF in culture and in stool samples. Overall, 113 bac...
متن کاملRapid detection of enterotoxigenic Bacteroides fragilis in diarrhoeal faecal samples.
BACKGROUND & OBJECTIVES Enterotoxigenic Bacteroides fragilis (ETBF) is an important aetiological agent of acute diarrhoeal illness in humans. It is a fastidious anaerobe and takes 3-5 days when grown in culture, and test for enterotoxin detection requires additional time conventionally. The present study was aimed to standardize a rapid and sensitive diagnostic assay and to screen the presence ...
متن کاملComparison of standard, quantitative and digital PCR in the detection of enterotoxigenic Bacteroides fragilis
Gut colonization with enterotoxigenic Bacteroides fragilis (ETBF) appears to be associated with the development of colorectal cancer. However, differences in carriage rates are seen with various testing methods and sampling sites. We compared standard PCR, SYBR green and TaqMan quantitative PCR (qPCR) and digital PCR (dPCR) in detecting the B. fragilis toxin (bft) gene from cultured ETBF, and f...
متن کاملRapid and sensitive assay for detection of enterotoxigenic Bacteroides fragilis.
Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and cytotoxic response in HT29/C1 cells. By using a pair of monoclonal antibodies ...
متن کاملDetection of enterotoxigenic Bacteroides fragilis in patients with ulcerative colitis
PURPOSE Ulcerative colitis (UC) as a type of inflammatory bowel disease (IBD), presumed to occur as a consequence of increased immune responses to intestinal microbiota in genetically susceptible individuals. Enterotoxigenic Bacteroides fragilis (ETBF) strains are important intestinal bacteria that can be involved in IBD. The aim of this study was to design a quantitative assay for detection of...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1997
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.35.10.2482-2486.1997